Real Time PCR Master Mixes
Alkali Scientific Radiant 2X HotStart Taq Mastermix, 40 Reactions,(1 x 1mL)
Radiant™ 2X Hot-Start Taq Mastermix Description RadiantTM 2x Hot-Start Taq Mastermix is a ready-to-use, robust PCR mix containing our highly purified Hot-Start Taq DNA Polymerase, reaction buffer, ultra-pure dNTPs, MgCl2 and PCR enhancers. RadiantTM 2x Hot-Start Taq Mastermix is optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA and crude samples. The Hot-Start technology is based on a new-generation PCR buffer formulation in conjunction with a proprietary antibody-mediated chemistry. RadiantTM Hot-Start Taq Mastermix exhibits 5´-3´ DNA polymerase activity with an error-rate of wild-type Taq ( 2.0 x 10-5). The polymerase mix is ideal for genotyping, colony PCR, multiplexing, low-copy assays, and the amplification of challenging targets susceptible to mispriming. RadiantTM Hot Start Taq Mastermix is engineered for robust, superior PCR and is supplied as a ready-to-use 2x mix which provides exceptional sensiti
Roche Diagnostics LIGHTCYCLER DNA MASTER H
LightCycler DNA Master HybProbe - 1 kit (for 96 reactions of 20 uL final reaction volume)
Roche Diagnostics FS ESSENTIAL DNA GREEN MASTER
FastStart Essential DNA Green Master Ready-to-use hot start reaction mix for SYBR Green I-based real-time PCR using the LightCycler 96 Instrument and the LightCycler Nano Instrument. 5 x 1 ml for up to 500 reactions of 20 l final volume
Sigma Aldrich Fine Chemicals Biosciences fastStart SYBR Green Maste
fastStart SYBR Green Master Instructions for UsefastStart(TM) SYBR(R) Green Master is a ready-to-use hot start reaction mix without ROX for quantitative polymerase chain reaction (qPCR) and reverse transcription (RT)-qPCR on real-time PCR systems other than the LightCycler(R) instruments. This master mix simplifies the preparation of reactions for DNA detection and analysis. In combination with a real-time PCR instrument suitable PCR primers and a hydrolysis probe fastStart(TM) TaqMan(R) Probe Master allows very sensitive detection and quantification of defined DNA sequences.SYBR(R) Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis the SYBR(R) Green I dye included in the reaction mix binds to the amplified PCR products. The amplicon can be detected by its fluorescence.Hot start protocols have been shown to significantly improve the specificity sensitivity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of fastStart(
Sigma Aldrich Fine Chemicals Biosciences fastStart Universal SYBR G
fastStart(TM) Universal SYBR(R) Green Master (Rox) is a ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX. SYBR(R) Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis the SYBR(R) Green I dye which is included in the reaction mix binds to the amplified PCR products. The amplicon can be detected by its fluorescence. Hot start protocols have been shown to significantly improve the specificity sensitivity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of fastStart(TM) Taq DNA Polymerase make the modified enzyme inactive at room temperature (15 to 25C). Therefore there is no elongation during the period when primers can non-specifically bind. fastStart(TM) Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e. a pre-incubation step at 95C).
INTACT GENOMICS INC FastAmp qRPA SYBR Kit, 25 Reactions
IG FastAmp qRPA SYBR kit that contains ready-to-use master mixes with all components except primers and template for isothermal amplification, detection, and quantification of DNA using Recombinase Polymerase Amplification. With this kit, RPA progress can be monitored in real-time and quantified using standardized template concentrations. The FastAmp qRPA SYBR kit is compatible with high throughput (96-well or 384-well) format fluorescent readers for faster results in just 30 minutes at constant 37⁰C. Now, primer sets can be validated by screening in parallel for isothermal RPA assay optimization with Flexible endpoint detection compatibility. Kit Includes: ,FastAmp qRPA SYBR Reaction Master Mix, IG RPA Enzyme Mixture v2 (4x), 280 mM Magnesium Acetate (MgOAc), Nuclease-free Water. Storage Temperature: -20°C
INTACT GENOMICS INC FastAmp qRPA SYBR Kit, 500 Reactions
IG FastAmp qRPA SYBR kit that contains ready-to-use master mixes with all components except primers and template for isothermal amplification, detection, and quantification of DNA using Recombinase Polymerase Amplification. With this kit, RPA progress can be monitored in real-time and quantified using standardized template concentrations. The FastAmp qRPA SYBR kit is compatible with high throughput (96-well or 384-well) format fluorescent readers for faster results in just 30 minutes at constant 37⁰C. Now, primer sets can be validated by screening in parallel for isothermal RPA assay optimization with Flexible endpoint detection compatibility. Kit Includes: ,FastAmp qRPA SYBR Reaction Master Mix, IG RPA Enzyme Mixture v2 (4x), 280 mM Magnesium Acetate (MgOAc), Nuclease-free Water. Storage Temperature: -20°C
New England Biolabs, Inc. NEBNext Q5 Hot Start HiFi PCR Master Mix – 50 reactions
NEBNext Q5 Hot Start HiFi PCR Master Mix is the original hot start NEBNext formulation of Q5 High-Fidelity DNA polymerase. This formulation is also included as a component in all of the original NEBNext Ultra kits, for DNA and RNA library preparation. However, the NEBNext Ultra II Q5 Master Mix is now available, and this more recent hot start formulation further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.
New England Biolabs, Inc. LunaScript RT SuperMix Kit – 25 reactions
LunaScript RT SuperMix Kit features an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It includes the thermostable Luna Reverse Transcriptase, which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is also included to protect template RNA from degradation. The LunaScript RT SuperMix contains random hexamer and poly-dT primers, allowing even coverage across the length of the RNA targets. In addition, the LunaScript RT SuperMix Kit contains a blue dye, providing a visual indicator that can be followed throughout the two-step RT-qPCR process. The LunaScript RT SuperMix offers robust, linear, and sensitive detection using total RNA inputs as high as 1 µg and as low as single copies of RNA.
New England Biolabs, Inc. Luna Universal Probe qPCR Master Mix – 2500 rxn (1 x 25 ml)
The NEB Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap fluorophores commonly used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR and existing as well as commercial qPCR assay primer/probe sequences can be used.
New England Biolabs, Inc. Luna Universal Probe qPCR Master Mix – 500 rxn (5 x 1 ml)
The NEB Luna Universal Probe qPCR Master Mix is a 2X reaction mix optimized for real-time qPCR detection and quantitation of target DNA sequences using hydrolysis probes. It contains Hot Start Taq DNA Polymerase and has been formulated with a unique passive reference dye that is compatible across a variety of instrument platforms (including those that require a high or low ROX reference signal). It also features dUTP for carryover prevention and a non-fluorescent, visible dye to monitor reaction setup. This dye does not spectrally overlap fluorophores commonly used for qPCR and will not interfere with real-time detection. The master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification and quantitation of DNA except primers/probes and DNA template. Genomic DNA or cDNA of interest can be quantitated with Luna qPCR and existing as well as commercial qPCR assay primer/probe sequences can be used.
Biotium Forget-Me-Not™ EvaGreen qPCR Master Mix (Low ROX) (500 reactions)
Forget-Me-Not™ EvaGreen qPCR Master Mix is a high performance, hot-start EvaGreen dye-based master mix for use in real time PCR applications and DNA melt curve analysis. Forget-Me-Not™ master mix has a light blue master mix color which helps prevent pipetting mistakes, saving time and reagents. The master mix features EvaGreen Dye which is suitable for HRM analysis and is safer, more environmentally friendly, and more sensitive than SYBR Green. The kit also features Cheetah™ Hotstart Taq for fast cycling protocols and Low ROX reference dye. This product has sufficient reagent for 500 PCR reactions.
Biotium Fast Plus EvaGreen qPCR Master Mix (trial size, 100 rxn)
Fast Plus EvaGreen qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Formulated using our environmentally safe EvaGreen dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq. This master mix is offered with ROX reference dye and has sufficient reagent for 100 reactions.
Biotium Forget-Me-Not™ EvaGreen qPCR Master Mix (2-Color Tracking) (500 reactions)
Forget-Me-Not™ EvaGreen qPCR Master Mix is a hot-start EvaGreen dye-based master mix for use in real time PCR applications and DNA melt curve analysis. Forget-Me-Not™ features a unique combination of a master mix containing a low concentration of blue dye, plus a DNA template buffer containing a higher concentration of blue dye. This 2-color tracking system allows you to see at a glance whether you forgot to add master mix or template to any of your reactions, so you can catch pipetting mistakes and avoid wasting time, reagents, and your precious DNA samples. The master mix features EvaGreen Dye which is suitable for HRM analysis and is safer, more environmentally friendly, and more sensitive than SYBR Green. The kit also features Cheetah™ Hotstart Taq for fast cycling protocols. This product has sufficient reagent for 500 PCR reactions.